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To mutate a residue follow these easy steps:
To mutate a residue follow these easy steps:
#Load a PDB file
<OL>
#Under the '''Wizard''' menu select '''Mutagenesis'''
<LI>Load a PDB file<BR><BR>
#In the PyMol viewer window select a residue
 
#Select '''No Mutation''' and select resultant residue
[[Image:Mutag1.png|180px|none]]
#Select Apply
<LI>Under the '''Wizard''' menu select '''Mutagenesis'''<BR><BR>
#Select Done
 
[[Image:Mutag1.png|thumb|1. Wizard -> Mutagenesis]]
[[Image:Mutag2.png|180px|none]]
[[Image:Mutag2.png|thumb|2. Select the Residue]]
<LI>In the PyMol viewer window select a residue<BR><BR>
[[Image:Mutag3.png|thumb|3. No Mutation; choose residue]]
 
[[Image:Mutag4.png|thumb|4. Apply; Done]]
[[Image:Mutag3.png|180px|none]]
<LI>Select '''No Mutation''' and select resultant residue<BR><BR>
 
[[Image:Mutag4.png|180px|none]]
<LI>Select Apply
<LI>Select Done
</OL>

Revision as of 20:40, 11 May 2006

Mutagenesis

PyMol has a Mutagenesis Wizard to make mutagenesis very easy for the end user.

To mutate a residue follow these easy steps:

  1. Load a PDB file

  2. Under the Wizard menu select Mutagenesis

  3. In the PyMol viewer window select a residue

  4. Select No Mutation and select resultant residue

  5. Select Apply
  6. Select Done
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