Biochemistry student intro

Biochemistry course

This tutorial was written directly for biochemistry students at Copenhagen University, 2012.

This is the very first introduction to the powerful molecular visualizer PyMOL.
We will only cover the very basic steps to get a image of your enzyme and put in your written student article.

Install PyMOL to your windows computer

You first want to install PyMOL.
Do this, by following this guide Pre-compiled_PyMOL
You don't have to follow the steps of extending PyMOL, but if you are a little technical, and want to become friends with PyMOL over time, then consider it.

Find a suitable protein data file

We need to find a Protein Databank File (PDB), which describe the x,y,z coordinates of your enzyme.
These are stored at the homepage: http://www.rcsb.org
Find a suitable file by searching for: porcine kidney fructose 1,6-bisphosphatase
The PDB file "1lev", seems suitable.

Read more about your protein

There exist homepages, to get more info about your protein.
These homepages collect material from several sources, and present them in easy format.

http://www.proteopedia.org/wiki/index.php/1lev
http://pdbwiki.org/wiki/1lev

Start your PyMOL

Start your shortcut to PyMOL "C:\Python27\PyMOL\PyMOL.exe"

Now click and do the following:

1. Top Menu: Plugin -> PDB Loader Service -> 1lev OK
2. Right Menu: -> 1lev -> "A" -> preset -> Publication
3. Top Menu: Display -> Sequence
4. Top Menu: Display -> Sequence Mode -> Chains
5. In Sequence, select so all "F" is marked.
6. Right Menu: -> (sele) -> "A" -> remove atoms
7. Top Menu: Display -> Sequence Mode -> Residue Codes
8. In Sequence, select so only substrate (F6P) is marked (A/338)
9. Right Menu: -> (sele) -> "A" -> zoom
10. Right Menu: -> (sele) -> "A" -> rename selection -> f6p
11. Right Menu: -> (f6p) -> "A" -> rename selection -> f6p
12. Right Menu: -> (f6p) -> "C" -> by element -> Select to Carbon is not green
13. In Sequence, select so only MN is marked (A/340)
14. Right Menu: -> (sele) -> "A" -> rename selection -> mn
15. Right Menu: -> (mn) -> "S" -> spheres
16. Write in console: select act_site, byres f6p around 3
17. Right Menu: -> (act_site) -> "S" -> sticks
18. Right Menu: -> (act_site) -> "L" -> residues
19. Find a good view, and push "Ray" in the top right of the grey command console.
20. Top Menu: File -> Save Image As -> PNG
21. Put it into your student article