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== Architecture == PySSA is written in pure Python and is among the first programs to utilize WSL2 in a production environment. It employs multiple instances of PyMOL, designated as User PyMOL, for direct user interaction with objects (proteins or protein pairs), and auxiliary PyMOLs, which are background instances utilized for tasks such as image rendering or distance analysis. The protein prediction is facilitated by ColabFold, which operates exclusively within the WSL2 environment. The WSL2 utilizes a specialized AlamaLinux distribution that includes ColabFold pre-installed.ColabFold communicates with a web server, MMSeqs2, to execute the MSA necessary for structure prediction. [[Image:PySSAArchitecture.png|left|400px|thumb|PySSA architecture on a local computer. The MSA generation for the ColabFold prediction is performed on a remote web server running the MMseqs2 software]]
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