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== How the script works == * The script integrate the Donor emission spectre, to divide with it. *: This is done by simple numerical integration by the [http://en.wikipedia.org/wiki/Rectangle_method Rectangle method] * All datapoints for the Acceptor excitation spectre are scaled, so that '''e'''(molar extinction coefficient) fits the wavelength where it is determined. * For the multiplication of the spectre, the script test that both data points exist before multiplication. *: This can be troublesome, if there is 1-2 nm between each data point. Or if they are "un-even". Like Donor has 100.2 100.4 100.4 and Acceptor has 100.1 100.3 100.5 *: But most spectre have much better resolution. Mostly 0.2 nm, and "even spaced" *: So this should work quite good as well. The output is a text file, with all the datapoints. # Column: Donor: The input Emission wavelength. # Column: Donor: The input Emission, arbitrary units of excitation. # Column: Donor: The input Emission, arbitrary units of excitation, divided by the integrated area. # Column: Acceptor: The input excitation wavelength. # Column: Acceptor: The input excitation '''e'''(molar extinction coefficient). # Column: Acceptor: The input excitation '''e'''(molar extinction coefficient), scaled correctly. # Column: The calculated overlap for this datapoint. # Column: The summed values for calculated overlap, until this datapoint. It also automatically generates a [http://www.gnuplot.info/ gnuplot] .plt file, for super fast making graphs of the spectres. In linux, gnuplot is normally part of the installation. Just write: gnuplot FILENAME.plt If you are in windows, [http://sourceforge.net/projects/gnuplot/files/ download gnuplot] and open the .plt file. Gnuplot makes three graphs in .eps format. They can be converted to pdf with the program: epstopdf or eps2pdf. They are for example part of LaTeX: C:\Program Files (x86)\MiKTeX 2.9\miktex\bin or you can [http://tinyurl.com/eps2pdf download it here]. The format of .eps is choses, since gnuplot then allows for math symbols in the graphs. <gallery heights="240px" widths="340px"> Image:1-ALEXA488Emi-ALEXA633Exi-overlap-all-spectre.png|All graphs plotted together, but not scaled correctly. Image:2-ALEXA488Emi-ALEXA633Exi-overlap-normalized-spectre.png|The Donor emission (y1) and Acceptor excitation (y2) scaled correctly. Image:3-ALEXA488Emi-ALEXA633Exi-overlap-integral.png|The Rectangle integral datapoint (y1) and the summed integration (y2). </gallery>
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